关键词: 柔红霉素;视网膜色素上皮;caspase-3;凋亡

    摘 要:目的 探讨天冬酰胺特异酶切的半胱氨酸蛋白酶(caspase)成员caspase-3在柔红霉素诱导的人视网膜色素上皮(RPE)细胞凋亡中的变化. 方法 原代培养的人RPE细胞经200μg L-1 柔红霉素诱导8，24和36h后，按照caspase-3荧光检测试剂盒说明处理细胞，再通过荧光比色法间接检测细胞碎片中caspase-3的活性. 结果 正常caspase-3的活性为(0.094±0.005)nmol AFC，8h后增加为(0.446±0.029)nmol AFC，24h后为(0.754±0.056)nmol AFC，36h又下降到(0.486±0.033)nmol AFC，但都高于正常水平(P<0.01).加入特异性四肽抑制物DEVD-CHO1μL后活性为(0.040±0.003)nmol AFC，显著低于正常(P<0.01). 结论 Caspase-3参与了柔红霉素诱导的人视网膜色素上皮细胞的凋亡，柔红霉素可使其活性增加.

    Caspase┐3activity during daunorubicin induced hu┐man retinal pigment epithelium cell apoptosis

    DU Hong-Jun，HUI Yan-Nian，WANG Yu-Sheng，HAN Quan-Hong，MA Ji-Xian

    Department of Ophthalmology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:daunorubicin;retinal pigment epithelium;cas-pase-3;apoptosis

    Abstract:AIM To study the function of caspase-3activity during daunorubicin induced human retinal pigment epitheli-um cell apoptosis.METHODS After being treated with200μmolL-1 daunorubicin for8，24and36h，the primary cul-tured human retinal pigment epithelium cells were treated by following the protocol of a specific assay kit ......