关键词: P物质;肢体负压;动脉闭塞性疾病;免疫组织化学

    中图号:R658

    摘 要:目的 观察肢体负压对周围动脉闭塞性病变犬皮肤中SP免疫反应阳性神经纤维的影响. 方法 犬17只，随机分治疗组10只、非治疗组5只和正常对照组2只，治疗组和非治疗组均将动物制作左后肢缺血模型，治疗组在模型制作后14d，开始行患肢负压治疗10d(15min·次-1 );非治疗组不做负压治疗;正常对照组不行缺血模型制作及负压治疗.3组均行左后肢趾皮肤免疫组化染色，检测SP免疫反应阳性纤维. 结果 非治疗组皮肤中SP免疫反应阳性神经纤维均较正常对照组明显增多(P<0.01)，治疗组较非治疗组减少(P<0.01)，但仍较正常对照组增多. 结论 肢体负压疗法可促进皮肤感觉神经纤维中SP的释放.

    Influence of limb negative pressure on cutaneous SP immunoreactive nerve fibers in dogs of peripheral arterial occlusive disease

    YI Jun，NING Mo-Fan，LI Juan，YANG Hua，QIU Jian-Yong

    Department of Vascular and Endocrine Surgery，Xijing Hospital，Department of Anesthesiology，Xijing Hospital，Institute of Neuroscience of Chi-nese PLA，Fourth Military Medical University，Xi'an710033，China

    Keywords:substance P;limb negative pressure;arterial occlusive disease;immunohistochemistry

    Abstract:AIM To observe the influence of limb negative pressure(LNP)on cutaneous SP immunoreactive nerve fibers in dogs with peripheral arterial occlusive disease.METHODS Seventeen dogs were divided into treatment(n=10)，nontreatment(n=5)and normal control(n=2)groups randomly.The left hindlimb ischemia model was made in nontreatment and treatment groups.The treatment group was treated by LNP for ten days(15min·d-1 )from the14th day after the models were made.However ......