关键词: 电磁脉冲;Fas;FasL;核酸杂交

    摘 要:目的 观察电磁脉冲对小鼠脾细胞Fas和FasL mRNA表达的影响. 方法 40只体质量(18±2)g的健康雄性BALB/c小鼠随机分为未照射组(8只)和全身照射组(32只).小鼠连续2d接受每日25次脉冲的电磁脉冲全身照射，每次照射间隔约2min.于照射后2，4，8和12h分别取2只未照射小鼠和8只照射小鼠的脾脏.采用RNA打点杂交法检测小鼠脾细胞Fas和FasL RNA的表达水平，并对杂交信号进行灰度扫描后计算RNA表达指数. 结果 受电磁脉冲照射后2h，小鼠脾细胞Fas和FasL的RNA表达指数即分别从未照射的0.59±0.26和0.56±0.23升高到0.97±0.25和0.96±0.21，4h又下降至0.75±0.24和0.69±0.22，8h再度上升达最高峰1.22±0.22和1.32±0.50(与对照组相比P<0.001)，于12h时下降至接近正常水平. 结论 电磁脉冲辐照可引起小鼠脾细胞Fas和FasL RNA表达水平波动性升高.

    Electromagnetic pulse increases mRNA expression of Fas and FasL in murine splenocytes

    LIU Jun-Ye，GUO Yao ，ZHENG Zhen-Xing，ZENG Gui-Ying，WANG Jin

    1 Department of Radiation Medicine，Faculty of pre-ventive Medicine，Fourth Military Medical Univer-sity，Xi'an710033，China，

    2 Institute of Northwest Nuclear Technology，Xi'an710024，China

    Keywords:electromagnetic pulse;Fas;FasL;nucleic acid hybridization

    Abstract:AIM To observe the effects of electromagnetic pulse on mRNA expression of Fas and FasL in murine splenocytes.METHODS Forty male Balb/c mice with body mass of(18±2)g were randomly designated non-irradiation group(8mice)and whole-body irradiation group(32mice).Mice received irradiation of electromagnetic pulse for two days ......