关键词: 表皮生长因子受体;c-erbB-2;基因表达;胶质瘤;原位杂交

    中图号:R739.41

    摘 要:目的 探讨表皮生长因子受体(EGFR)和c-erbB-2基因表达与人脑胶质瘤发生发展的关系. 方法 采用原位杂交方法检测52例人脑胶质瘤、2株人脑胶质瘤细胞系及8例正常人脑组织中EGFR和c-erbB-2基因mRNA的表达. 结果 EGFR mRNA阳性率在高恶性度(Ⅲ～Ⅳ级)胶质瘤为79.2%，低恶性度(Ⅰ～Ⅱ级)胶质瘤为50.0%，二者差异显著(P<0.05);c-erbB-2mRNA阳性率在高恶性度胶质瘤为83.3%，低恶性度胶质瘤为71.4%，二者无显著差别(P>0.05);EGFR和c-erbB-2mRNA表达均随胶质瘤恶性程度增高而增强(P<0.01).EGFR和c-erbB-2mRNA在2株人脑胶质瘤细胞系中均有表达，在8例正常人脑组织中均无表达.EGFR和c-erbB-2mRNA表达结果密切相关(P<0.05). 结论 EGFR和c-erbB-2基因在人脑胶质瘤中普遍表达，且随胶质瘤恶性程度增高而表达增强，EGFR和c-erbB-2基因共表达在人脑胶质瘤发生发展过程中发挥重要作用.

    Expression of EGFR and c┐erbB┐2gene in human gliomas:An in situ hybridization study

    ZHEN Hai-Ning，ZHANG Xiang，ZHANG Zhi-Wen，FEI Zhou，ZHANG Jian-Ning，WU Jing-Wen，CAO Wei-Dong，QUYan，ZHANG Yong-Qin

    Institute of Neurosurgery of Chinese PLA，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:epidermal growth factor receptor;c-erbB-2;gene expression;glioma;in situ hybridization

    Abstract:AIM To research the role of expression of epider-mal growth factor receptor(EGFR)and c-erbB-2genes in the initiation and progression of human gliomas.METHODS The expression of EGFR and c-erbB-2genes was investigated by using in situ hybridization in52cases of gliomas ......