关键词: 丝裂霉素C;人结膜囊成纤维细胞;胶原合成;细胞培养

    中图号:R776.1

    摘 要:目的 观察丝裂霉素C(MMC)对培养的人结膜囊成纤维细胞胶原合成的影响. 方法 用3 H-脯氨酸掺入和原位杂交法观察MMC对培养人结膜囊成纤维细胞胶原合成活性的作用. 结果 0.4g·L-1 MMC作用5min，可使结膜囊成纤维细胞对3 H-脯氨酸的摄取减少55%以上(P<0.05)，并降低了Ⅰ型前胶原mRNA原位杂交信号. 结论 临床青光眼滤过手术中使用的MMC剂量与作用时间，可减少成纤维细胞胶原的合成，有利于青光眼滤过手术成功率的提高.

    Mitomycin C acts on collagen synthesis of human Tenon's capsule fibroblasts

    HU Dan，HUI Yan-Nian，Philip P.Chen

    Department of Ophthalmology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China，Department of Ophthalmology，University of Washington，Box356485，Seattle，WA98195-6485，USA

    Keywords:mitomycin C;human Tenon's capsule fibroblast;collagen synthesis;cell culture

    Abstract:AIM To investigate the effects of mitomycin C(MMC)on the collagen synthesis in human Tenon's capsule fibroblasts.METHODS The cultured human Tenon's fi-broblasts were exposed to MMC.3 H-proline incorporation was performed to determine the collagen production ......