关键词: 高压氧;脊髓;缺血再灌注损伤;自由基;缺血耐受

    中图号:R651.2

    摘 要:目的 探讨高压氧预处理诱导脊髓缺血耐受的机制. 方法 21只雄性新西兰大白兔，随机分为3组(每组n=7):对照组，即常压空气组;HBO组，即单纯高压氧(HBO)预处理组(100%O2 ，253.3kPa，1h d-1 ，5d);DMTU组，HBO预处理条件同HBO组，但于每次HBO处理前1h予500mg kg-1 二甲基硫脲(DMTU)iv.最后一次HBO处理后24h采用肾下主动脉阻断法造成脊髓缺血(20min)，观察再灌注后48h时的神经功能评分，及脊髓(L5 )病理学变化.并同时测定后两组动物在HBO预处理前24h，每次HBO处理后1h，脊髓缺血前10min和再灌注10min时的血中丙二醛(MDA)水平. 结果 再灌注48h时神经功能评分HBO组明显优于对照组和DMTU组(P<0.05);脊髓前角正常运动神经元计数明显多于对照组和DMTU组(P<0.05).MDA水平各时间点两组间无显著差别. 结论 高压氧预处理所诱导的脊髓缺血耐受的机制与氧自由基的产生有关.

    Mechanism of hyperbaric oxygen preconditioning┐induced ischemic tolerance in spinal cord of rabbits

    XIONG Li-Ze，DONG Hai-Long，ZHU Zheng-Hua，LOU Li-Chao，XIONG Dong-Fang，LU Zhi-Hong，CHEN Min

    Department of Anesthesiology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:hyperbaric oxygenation;spinal cord;ischemia-reperfusion injury;free radicals;ischemic toler-ance

    Abstract:AIM To investigate the mechanism of hyperbaric oxygen(HBO)preconditioning-induced ischemic tolerance in spinal cord.METHODS 21male New Zealand white rabbits were randomly allocated to one of3groups(7in each group):animals in control group received room air and nor-mal ambient pressure(1h d-1 ......