关键词: 烧伤;多器官功能衰竭;兔

    摘 要:目的 制备兔烫伤早期多器官功能衰竭(MOF)模型. 方法 兔乙醚吸入法全身麻醉后，以95℃水烫伤其背部及躯干两侧30s，造成30%体表面积III度烫伤(III度30%TBSA).烫伤后48h，用戊巴比妥钠耳缘静脉注射(30mg·kg-1 )麻醉兔，检测假烫对照组及烫伤组48h后的平均动脉血压、中心静脉压、心输出量，计算总外周阻力、心指数;测定肺泡灌洗液蛋白及血浆总蛋白，计算肺通透指数;测定动脉血氧分压、氧饱和度，测定血中肌酸磷酸激酶、谷丙转氨酶、尿素、肌酐、血小板等反映心、肺、肝、肾、血液功能的一系列指标. 结果 烫伤后48h兔心、肝、肾功能下降达到衰竭标准，肺功能下降，总外周阻力上升，动物发生MOF. 结论 利用兔可制备烫伤早期MOF模型;MOF可在动物血压正常时发生.

    Preparation of rabbit multiple organ failure model in early postburn

    LIU Ya-Ling LIN Shu-Xin XU Ming-Da LI Zhi-Chao CHEN Bi ZHANG Shi-Fan

    1 Department of Pathophysiology，Faculty of Pre-clinical Medicine，

    2 Department of Burn Surgery，Center of Plastic Surgery，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China，

    3 Department of Cardiothoracic Surgery，The Gener-al Hospital of Lanzhou

    Keywords:burns;multiple organ failure;rabbits

    Abstract:AIM To establish model of rabbit multiple organ failure(MOF)in early postburns.METHODS All rabbits were anesthetized with ether inhalation.The dorsal skin of rabbits were immersed in95℃water(or37℃water)for30s.This procedure produced a full thickness burn of30%total body surface area(TBSA)or sham burn.At48h postburn ......