影响家蚕表达外源基因效率的几个重要因子的比较研究
家蚕,,家蚕;重组家蚕核型多角体病毒;基因表达;表达效率;影响因子,1材料与方法,2结果,3讨论,参考文献
摘 要: 本研究以重组病毒rSj14NPV为感染病毒,通过比较纯化蛋白的产量,对家蚕作为生物反应器生产基因工 程产品的适宜蚕品种与相关的重组病毒接种技术进行了比较研究。研究结果表明:871×872、秋风×白玉为表达 Sj14的适宜蚕品种;重组病毒接种量与蛋白表达量有关,适宜接种量为4~5μL/蚕;适宜接种时间以5龄2日龄为 优;以家蚕表达外源基因,最好将重组病毒以家蚕细胞复苏后再接种家蚕以保证获得较高的表达效果。关键词: 家蚕;重组家蚕核型多角体病毒;基因表达;表达效率;影响因子
COMPARATIVE STUDIES ON SOME IMPORTANT FACTORS INFLUENCING THE GENE EXPRESSION EFFICIENCY IN THE SILKWORM EXPRESSION SYSTEM
LIU Jin-ming, FU Zhi-qiang, LIN Jiao-jiao, CAI You-min, GUI Zhong-zheng , ZHUANG Da-huan, WU Xiang-fu
(1. Shanghai Research Center for Animal Biotechnology, Shanghai Institute of Domestic Animal Parasitology, CAAS, Key Laboratory of Animal Parasitology, Ministry of Agriculture, Shanghai 200232, China; 2. The Sericultural Research Institute, CAAS, Zhenjiang 212018, China; 3. Shanghai Institute of Biochemistry, CAS, Shanghai 200031, China)
Abstract: Comparative studies on some important factors influencing the gene expression efficiency in the silkworm expression system ......
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