【摘要】 目的 探讨胆红素脑病时不同浓度、时间脑损伤的脑源性神经营养因子(BDNF)表达变化情况。方法 制作100mg/kg、200mg/kg豚鼠胆红素脑病模型，分别在给予胆红素4h、8h后处死，采用免疫组织化学染色法检测脑组织内BDNF阳性神经元的数量。结果 100mg/kg、200mg/kg 4h的BDNF阳性神经元数量稍少于正常对照组，但差异无显著性，8h的BDNF阳性神经元数量明显多于正常对照组和4h组，差异有显著性。结论 BDNF在胆红素脑病早期，BDNF稍少，可能是：(1)在神经保护和修复中消耗大量BDNF，短期内代偿不足；(2)胆红素脑病初期是可逆的。不足以刺激神经元产生大量BDNF，随着损伤和时间延长。BDNF表达明显增高，有对抗损伤和修复受损神经元作用。

    【关键词】 胆红素脑病；脑源性神经营养因子；免疫组织化学染色法；表达

    A study on expressional change of brain-derived neurotrophic factor in newborn rat bilirubin encephalopathy

    HE Ping,WANG Ting-hua,DU Kai-Xian,et al.

    The Second Affiliated Hospital of Kunming Medical College,Kunming 650101,China

    【Abstract】 Objective To study expressional change of BDNF in newborn rat bilirubin encephalopathy.Methods Newborn rat (4～6d after birth) with bilirubin encephalopathy were induced by administration intra-peritoneally with bilirubin (100mg/kg,200mg/kg),using immunohistochemical reaction to detect BDNF.Results (100mg/kg,200md/kg)after 4 h,BDNF positive neurons are little decreased,after 8 h,BDNF positive neurons are much more increased.Both that are compared with normal newborn rat.Conclusion In early bilirubin encephalopathy.BDNF expression are little decreased(P>0.05),in latter bilirubin encephalopathy.BDNF expression are much more increased(P<0.01). ......