肝炎病毒__(期刊论文)

HBVTR重组腺病毒载体的构建

http://www.100md.com 《第四军医大学学报》 2004年第4期

肝炎病毒,,肝炎病毒，乙型；靶向核糖核酸酶；腺病毒载体,0引言,1材料和方法,2结果,3讨论,【参考文献】

     ·研究原著·

    Construction of the HBVTR recombinant adenovirus vector

    ZHAO Ya, GONG WeiDong, LIU Jun, LI YingHui, DING Jin, XUE CaiFang

    Department of Etiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To construct the recombinant adenovirus vector of HBV targeted ribonuclease (HBVTR) gene. METHODS: HBVTR gene and the control groups genes were cloned into plasmid pDC316, respectively, to construct shuttle plasmid pDC316/TR and the control shuttle plasmids. Then the obtained shuttle plasmids were cotransfected with rescue plasmid pBHGlox (delta) E1, 3Cre into HEK293 cells to acquire recombinant adenovirus Ad/TR and control vectors. The recombinant adenoviruses titers were measured by means of plaque assay. RESULTS: Recombinant adenovirus vector of HBVTR gene was successfully constructed and high titers of recombinant adenoviruses were obtained. CONCLUSION: Construction of the HBVTR recombinant adenovirus is successful.

    【Keywords】 hepatitis B virus; targeted ribonuclease; adenovirus vector

    【摘要】目的：构建乙肝病毒靶向核糖核酸酶(HBV targeted ribonuclease, TR)基因的重组腺病毒载体. 方法：将HBVTR基因及其对照组基因分别克隆入质粒载体pDC316得到pDC316/TR等穿梭质粒 ......

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