Establishment of immunosuppressed mice subrenal capsular model and examination of microvessel density

    WANG Yu, TIAN Jing, LI Ke, LI Yan, YAO Minª²Jie

    1Department of Clinical Laboratory, 2Department of Physiology, Jilin Military Medical College, Fourth Military Medical University, Jilin 132013, China, 3No. 306 PLA Hospital, Beijing 100000, China

    ¡¾Abstract¡¿ AIM: To establish a mouse subrenal capsular model and to determine its roles in tumor angiogenesis. METHODS: BALB/c mice were pretreated with CTX to establish the immunosuppressed mice model and SRCA (subrenal capsular assay) was carried out simultaneously with fresh explants of human gastric cancer in two groups: the CTXª²immunosuppressed mice and normal mice. Microvessel density was examined by immunohistochemical staining with antihuman factor ¢ø antibody. RESULTS: The xenografts grew constantly within 7 days after SRCA in CTX mice and the xenograft size in the CTX mice was bigger than that in normal mice (P<0.05) and the microvessel density in the CTX mice was significantly higher than that in the normal mice(P<0.05). CONCLUSION: Mouse subrenal capsular transplantation is an acceptable shortª²term in vivo model in the study of tumor angiogenisis. Xenografts under renal capsule of CTX immunosuppressed mice grow constantly within 7 days and angiogenisis is observed. ......