era基因_____小鼠；era基因；基因表达；蛋白质类

小鼠ERA蛋白在大肠杆菌中的表达及其纯化

http://www.100md.com 《第四军医大学学报》 2004年第8期

era基因,,小鼠；era基因；基因表达；蛋白质类,分离和提纯,0引言,1材料与方法,2结果,3讨论,【参考文献】

     Expression and purification of mouse ERA protein in Escherichia coli

    DU MengGang, CHEN SuMin, WU YuanMing, DONG Ke, CHEN NanChun, ZHENG Yu

    Department of Biochemistry & Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To express and purify mouse ERA protein for the further study of eukaryotic ERA function. METHODS: The fused protein expression vector pMSMmERA was constructed. The MBPmERA was expressed in E. coli after induction and the target protein was purified by means of amylose affinity chromatography and identified by Western blotting. RESULTS: The expressed MBPmERA protein accounted for 18% of the total bacterial protein and was identified by Western Blotting. The purity of the fused protein was 70 % after amylose affinity chromatography. CONCLUSION: The fused mera gene can be expressed with high efficiency in E.coli by gene recombination technique and the fused protein is purified preliminarily. ......

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