酵母双杂交_____核蛋白MINT;

核基质MINT N端片段(365~1084aa)相互作用蛋白的筛选及鉴定

http://www.100md.com 《第四军医大学学报》 2004年第1期

酵母双杂交,,核蛋白MINT;,MSX2;,RBPJκ;,Notch信号途径;,酵母双杂交,核基质MINTN端片段(365~1084aa)相互作用蛋白的筛选及鉴定,0引言,1材料和方法,2结果

     Screening and identification of nuclear matrix protein MINT Nterminal fragment (365-1084aa) interacting proteins

    QIN Hong, SUN Qiang, ZHOU Peng, LI JunFeng, HAN Hua, LUO XiaoXing

    1Departerment of Medical Genetics and Development Biology, 3Departerment of Pharmacology, School of Basic Medicine, Fourth Military Medical Univercity, Xi’an 710033, China, 2For Stem Cell Center, Tangdu Hospital, Fourth Military Medical Univercity, Xi’an 710038, China

    【Abstract】 AIM: To identify the function and mechanism of nuclear matrix protein MINT (Msx2interacting nuclear target protein). METHODS: Yeast twohybrid assay was used to screen proteins that interact with a fragment of MINTF2 (365-1084aa). From 1.0×108 yeast clones transformed with the bait plasmid and a cDNA library of 9 dpc mouse embryo,128 were positive for nutritional screening and βgalactosidase assay. Restriction digestion showed 4 independent positive clones and they were analyzed by DNA sequencing, and revealed that they represent correctly fused cDNA fragments. RESULTS: Screened 4 proteins that interacting with MINTF2, which are 67 ku laminin receptor, two of 16 S ribosomal RNA, ArginyltRNA synthetase. CONCLUSION: Succeed screening of interacting with MINTF2 proteins, and made the basement for further study of the function and mechanism of MINT. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/02/19/87/14/25.htm

您现在查看是摘要页，全文长 10123 字符。