乙酰肝素酶_____乙酰肝素酶；硫酸乙酰肝素；基因工程；

人肝癌组织中乙酰肝素酶基因的克隆与序列分析

http://www.100md.com 《第四军医大学学报》 2004年第1期

乙酰肝素酶,,乙酰肝素酶；硫酸乙酰肝素；基因工程；,肝癌细胞,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Cloning and sequencing of human heparanase gene from hepatoma

    ZHENG RenHong, WANG WeiZhong, LIANG KeMing

    1Department of Gastrointestinal Surgery, 2Department of Radiation Therapy, Xijing Hospital, Fouth Military Medical University, Xi’an 710033, China

    【Abstract】 AIM: To study the role of heparanase in the growth and metastasis of human hepatocelluar carcinoma (HCC), and to colone heparanase cDNA gene from HCC. METHODS: The cDNA fragment encoding heparanase was isolated by RTPCR method with the total RNA extracted from human hepatoma, linked into pGEMT easy vector, then amplified, cloned, finally the recombinant plasmids was identificated by Electrophoresis. RESULTS: The result of sequencing indicated that the sequences of our cDNA was identical to the corresponding parts of the mRNA for human heparanase in GenBank except 16 nucleotides, and with a homoloty of 0.991. CONCLUSION: Human heparanase cDNA gene is successfully cloned from HCC.

    【Keywords】 heparanase; sulfate heparan; genetic engineering; hepatoma

    【摘要】目的: 研究乙酰肝素酶在人肝癌细胞生长和转移中的作用 ......

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