肿瘤_____肿瘤；抗药性；NFκB；脱噬作用

NFκB抗凋亡介导肺腺癌群集耐药

http://www.100md.com 《第四军医大学学报》 2004年第10期

肿瘤,,肿瘤；抗药性；NFκB；脱噬作用,0引言,1材料和方法,2结果,3讨论,【参考文献】

     NFκB mediates multicellular drug resistance of lung adenocarcinoma by antiapoptosis

    HE JianMing, LIANG HouJie, HUANG HaiHui, HU ShaoYi, BIAN ZhiHeng

    Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China

    【Abstract】 AIM: To explore the role and probable mechanism of NFκB signal transduction in the multicellular drug resistance of lung adenocarcinoma. METHODS: Multicellular spheroids (MCS) were employed to simulate cellcell adhesion in the malignant mass. We compared the sensitivity of MCS to Adriamycin ( ADM ) with that of monolayer cells (MC) before and after blocking the activity of NFκB by adding NFκB inhibitor SN50. The activity of NFκB was analyzed by electrophoretic mobility shift assay and apoptosis was detected by flow cytometry and PI (propidiumiodium) staining. Bcl2 and BclxL expression levels were detected by flow cytometry and indirect immunofluorescent staining. RESULTS: Compared with MC, MCS had more than two layers of cells and had more extensive and compact cell adhesion. MCS were more resistant to adriamycin, and their NFκB activity, Bcl2 and BclxL expressions were much higher. After treatment with adriamycin (ADM), the NFκB activity of the cells increased. When NFκB was blocked, cells, especially those grown as MCS, became more sensitive to ADM and apoptosis increased while Bcl2 and BclxL expression decreased. CONCLUSION: NFκB plays an important role in multicellular resistance by upregulating BclxL and Bcl2 and suppressing apoptosis. ......

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