Experimental study on model of pancreas transplantation alone with enteric drainage in rats

    LIU Xiaoª²Nan, WANG Weiª²Zhong, LI Kaiª²Zong, GUAN Wenª²Xian, CHEN Caiª²Ping, CHEN Dongª²Li, LUO Lan

    1Department of Gastrointestinal Surgery, 2Department of Hepatobiliary Surgery, 3Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To establish the rat model of pancreas transplantation alone (PTA) with enteric drainage. METHODS: Inbred SD rats were randomly assigned into 2 groups: a group of 10 nondiabetic rats as control and a group of 22 diabetic rats, which received PTA from the normal donors. Diabetic rats were induced by streptozotocin. Venous drainage of PTA was performed using endª²toª²side anastomosis between the donors¬ð abdominal aorta and recipients¬ð abdominal aorta segment, and endª²toª²end anastomosis between the donors¬ð portal vein segment and recipientsª² left renal vein (with a cuff). Pancreas exocrine drainage was made by pancreas intestine anastomosis (Rouxª²Y). The blood glucose, body weight, food intake, water intake and urine volume were monitored before transplantation and on the 1st, 3rd, 7th, 14th and 30th day after transplantation. RESULTS: The mean duration of operation of PTA group in donors was (32.2¡À12.7) min, and that in recipients was (63.4¡À15.9) min, and the mean duration of cold ischemic time was 0 min and that of warm ischemic time was (48.6¡À18.3) min. 11 (50%) of the 22 cases in PTA group died or lost the endocrine function within 1 month. The main complications were pancreastitis and pancreas leak after transplantation (7 cases, 31.8%). The blood glucose of all successful recipients lowered on the first day after transplantation and restored to normal on the third day. The food intake, water intake and urine volume decreased and became stable after 14 days. CONCLUSION: Successful PTAs can restore the pancreatic endocrine function of the diabetic rats. ......