金属蛋白酶类__(期刊论文)

GFR/DDR2嵌和受体的构建和稳定表达的鉴定

http://www.100md.com 《第四军医大学学报》 2004年第12期

金属蛋白酶类,,盘状结构域受体；嵌和受体；金属蛋白酶类；基因表达,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Construction and stable expression of EGFR/DDR2 chimeric receptor

    SU Jin, YU JiangTian, WANG JiCun, SHI Man, ZHU Ling, ZHANG Jian, LIU XinPing, YAO LiBo

    Department of Biochemistry & Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract 】 AIM: To analyze DDR2 signal pathway by avoiding activating a related family member with the same ligand as DDR2. METHODS: The EGFR/DDR2 chimeric receptor was fused by PCR amplification and enzyme digestion and then stably transfected to 293 cell line with relatively low EGFR expression. After screening with G418, positive clones that stably express this chimera on the membrane were identified by flow cytometry (FCM). RESULTS: EGFR/DDR2 chimeric expressing plasmid was successfully constructed and the clone stably expressing the plasmid was screened. CONCLUSION: The EGFR/DDR2 chimeric receptor has been fused and stably expressed.

    【Keywords 】 dicordin domain receptor; chimeric receptor; metalloproteinases; gene expression

    【摘要】目的：将一在细胞膜表面不表达或低表达的受体胞外区与盘状结构域受体2(DDR2)的胞内区融合 ......

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