Phosgeneª²induced oxidative injury in rats and effects of NaHCO3 buffer on it

    LIANG Xin, WANG Peng, HAI Chunª²Xu

    Department of Toxicology, School of Preventive Medicine, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To probe into phosgeneª²induced oxidative injury in rats and the effects of NaHCO3 buffer on it and to investigate the mechanism of pulmonary edema formation induced by phosgene and the therapy for phosgene poisoning. METHODS: Forty animals were randomly divided into four groups: control group, phosgene group, 0.2 mL NaHCO3 buffer group and 0.5 mL NaHCO3 buffer group. The phosgene, 0.2 and 0.5 mL groups were exposed to phosgene air (11.9 g/m3¡Á3 min ) and were injected with NaHCO3 buffer intraperitoneally at the fifth minute. The third hour after exposure, all rats were killed by decollation. Fluorescence was employed to measure MDA, GSH and GSSG of the right lung, serum and liver and the left lung wetª²toª²dry weight ratio was calculated. RESULTS: The difference in the lung MDA, serum MDA, GSH and GSSG, and liver GSSG were significant between the control group and phosgene group. Compared with those of phosgene group, the lung MDA and the serum GSSG and MDA of 0.5 mL NaHCO3 buffer group were significantly different. The serum GSH and liver GSSG of 0.2 mL NaHCO3 were also significantly different compared with those of phosgene group. CONCLUSION: NaHCO3 buffer may have relieving effects on oxidative system damage induced by phosgene exposure. ......