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    野葛愈伤组织诱导与不定芽分化

    王义强1

    , 唐隆平2

    , 蒋舜村2

    , 陈喜龙1

    , 郑 雨1

    (1. 中南林学院生命科学与技术学院, 湖南 株洲 412006; 2. 湖南省东安舜皇山森林公园, 湖南 永州 425000)

    [摘 要] 采用M S 为基本培养基, 附加NAA、 62 BA 和 IAA 3 种激素, 诱导野葛不同外植体愈伤组织形成; 再将愈伤组织接种在不

    同浓度62 BA 的M S 培养基上, 分别在光照和黑暗条件下进行不定芽的诱导。结果表明, 野葛幼叶、茎段和顶芽在适宜激素的诱导下均

    能形成愈伤组织, 但不同激素组合其愈伤组织诱导率不同。幼叶愈伤组织诱导的最适培养基为M S+ NAA 1. 0 mg? L + 62 BA 1. 0

    mg? L , 其诱导率为75% , 茎段愈伤组织诱导的最适培养基为M S+ NAA 1. 0 mg? L + 62 BA 3. 0 mg? L + IAA 0. 2 mg? L , 其诱导率为

    70%。不同外植体形成的愈伤组织其不定芽的分化诱导率不同, 茎段愈伤组织在不同浓度62 BA 下均能诱导出不定芽; 光照有利于芽

    的分化, 在光培养条件下, 茎段愈伤组织不定芽平均诱导率为66. 7%。

    [关键词] 野葛; 组织培养; 愈伤组织; 不定芽

    [中图分类号] S58; S604+ . 3 [文献标识码] A [文章编号] 1003- 8981 (2004) 01- 0019- 03

    Ca llus Inducemen t and Bud D if feren t ia t ion in Puera r ia loba ta

    WANGY i -Qiang

    1

    , TANGLong-P ing

    2

    , J IANG Shun-Cong

    2

    , CHEN Xi -Long

    1

    , ZHENGYu1

    (1. Co llege of L ife Sciences and Techno logy, Cent ral South Fo rest ry U niversity, Zhuzhou 412006, Hunan, Ch ina;

    2. Dong’an Shunhuangshan Fo rest Park, Yongzhou 425000, Hunan, Ch ina)

    Abstract : Callus inducement and advent it ious bud different iat ion in P ueraria lobata w ere car r ied out by cultur ing on

    M S w ith ho rmones NAA , 62 BA , and IAA. Results indicated that young leaves, stem f ragments and t ip buds could

    all be induced into callus under suitable ho rmones, but the inducement rate var ied w ith different ho rmone

    combinat ions . The best medium fo r callus fo rmat ion f rom young leavesw asM S+ NAA 1. 0mg? L + 62 BA 1. 0mg? L ,and the rate of callus fo rmat ion w as 75 percent; the best medium fo r callus fo rmat ion f rom stem sect ions w asM S+

    NAA 1. 0 mg? L + 62 BA 3. 0 mg? L + IAA 0. 2 mg? L , and the rate of callus fo rmat ion w as 70 percent . The rate of

    advent it ious bud different iat ion var ied w ith the calli induced f rom different exp lants, and the advent it ious buds could

    be induced f rom the calli fo rmed f rom stem sect ionsw ith var ious concent rat ions of 62 BA. L igh t w as favo rable fo r the

    bud different iat ion, and under ligh t condit ions, the average rate of bud fo rmat ion fo rm stem sect ions w as 66. 7

    percent .

    Key words : P ueraria lobataW illd . ; t issue culture; callus; advent it ious bud

    野葛P uera ria loba ta 为豆科葛属多年生落叶藤本植物, 具有多种经济价值, 主产湖南、广东、福建、云南、贵州、四川、安微等省(区)

    [ 1 ]。我国医学认为, 野葛性凉、味甘、辛, 入脾、胃经[ 2 ]。近年来, 随着科技的不断发展,国内外医学工作者对野葛的进一步深入研究表明, 异黄酮类化合物是野葛的主要有效活性成分, 干燥野葛中总

    黄酮含量高达 5%～ 10% [ 3 ] ......