脱氧寡核苷酸__(期刊论文)

99mTcPDGFRβ AODN的制备及其生物分布

http://www.100md.com 《第四军医大学学报》 2004年第24期

脱氧寡核苷酸,,99mTc；生物学标记；脱氧寡核苷酸，反义；生物分布,99mTcPDGFRβAODN的制备及其生物分布,0引言,1材料和方法,2结果,3讨论

     Preparation and biodistribution of 99mTcPDGFRβ AODN

    CHENG ShiWu，HOU YingPing，LIU ChangBin，WANG Yi，WANG Zhe，DENG JingLan

    1Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xian 710033,China，2College of Chemistry & Molecular Engineering, Peking University, Beijing 100871, China

    【Abstract】AIM： To explore a better method of radiolabeling platelet derived growth factor receptor-β(PDGFRβ) antisense oligodeoxynucleotide (AODN) with 99mTc and to investigate the biodistribution of radiolabeled AODN in mice. METHODS: A 18mer singlechain AODN, complementary to PDGFRβ mRNA, was conjugated with chelator NHSMAG3 and labeled with 99mTc. The stability of 99mTcMAG3AODN in vitro was investigated and biodistribution was studied in normal mice. The labeling efficiency was measured by paper chromatography. RESULTS: As proved by paper chromatography and P4 column analysis, AODN was successfully radiolabeled by 99mTc. The results showed that the average labeling efficiency reached 70%. In saline at room temperature and in serum at 37℃, the radiochemical purity of 99mTcMAG3AODN was higher than 90% in 24 hours. The biodistribution study in normal mice showed that there was a higher uptake in kidney and liver. CONCLUSION: It is concluded that these strategies for labeling AODN with 99mTc are simple and efficient. 99mTcMAG3AODN is stable in vitro and appears to be suitable for further experiments and therapeutic applications in the future. ......

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