关键词: 成纤维细胞生长因子，碱性;肌，骨骼/细胞学;细胞低氧;基因，Bc1-2

    摘 要:目的 观察碱性成纤维细胞生长因子(bFGF)对缺氧复氧(A/R)损伤后肌细胞凋亡(apoptosis)相关基因蛋白Bc1-2表达的影响. 方法 将培养的骨骼肌细胞分为正常对照组、A/R组及浓度分别为10，20和40μg L-1 的bFGF预处理加A/R组共5组，然后用免疫组化方法(ABC法)进行染色，统计分析Bc1-2阳性的肌细胞百分率、平均吸光度的变化. 结果 经bFGF预处理后，Bc1-2阳性的肌细胞百分率(如bFGF20μg L-1 组与对照组相比，23.3%→34.7%，P<0.01)、平均吸光度(如bFGF20μg L-1 组与对照组相比，0.13→0.20，P<0.01)均显著增加. 结论 bFGF对A/R损伤的骨骼肌细胞的凋亡可能具有抑制作用.

    Effect of bFGF on Bcl┐2expression in anoxia and reoxygenation injured cultured skeletomyocytes

    MA Yan SUN Yue-Fen GAO Wei ZHOU Chang-Man

    1 Department of Medicine，

    2 Experimental Center of Neural Biology，Beijing Military Medical College，Beijing100071，China

    Keywords:fibroblast growth factor，basic;muscle，skeletal/cytology;cell hypoxia;genes，Bcl-2

    Abstract:AIM To observe the Bcl-2expression on the cul-tured skeletal muscle cells in neonatal rats injuried by anoxia/reoxygenation(A/R)and preconditioned by basic fibroblast growth factor(bFGF).METHODS We divided cultured skeletomyocytes into five groups ......