关键词: 色素上皮，眼;细胞培养;增殖;苏拉明;四甲基偶氮唑盐比色法;细胞分裂指数;AgNORs染色

    摘 要:目的 研究苏拉明(suramin)对培养人视网膜色素上皮细胞(retinal pigment epithelium，RPE)增殖的影响. 方法 将不同质量浓度的苏拉明(1.5，15和150mg·L-1 )加入RPE细胞培养液，采用四甲基偶氮唑盐(tetrazolium，MTT)比色法，细胞分裂指数计数和核仁组成区嗜银染色(AgNORs)检测苏拉明对RPE增殖活力的影响. 结果 含有100mL·L-1 小牛血清的培养液可以显著刺激RPE的增殖(P<0.01)，无血清组A值为0.19±0.01、含血清组A值为0.30±0.01;苏拉明抑制了100mL·L-1 血清条件下RPE的增殖，呈剂量依赖性，最大抑制率达51%，3种浓度组A值分别为0.29±0.01，0.24±0.01和0.14±0.01，对细胞的形态无明显影响. 结论 苏拉明对血清刺激RPE增殖有显著非毒性抑制作用.

    Effects of suramin on proliferation of cultured hu┐man retinal pigment epithelial cells in vitro

    KANG Jun，HUI Yan-Nian，CUI Zhi-Li，HAN Quan-Hong

    Departmant of Ophthalmology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:pigment epithelium of eye;cell culture;prolifera-tion;suramin;MTT;cell division index;Ag-NORs

    Abstract:AIM To investigate the effects of suramin on pro-liferation of cultured human retinal pigment epithelial(RPE)cells in vitro.METHODS The cultured human RPE cells were added with suramin(1.5 ......