关键词: 维甲酸;苯乙酸钠;肝细胞癌;细胞分化;甲胎蛋白

    摘 要:目的 探讨全反式维甲酸(ATRA)和苯乙酸钠(Na-PA)两者对人肝癌细胞联合作用. 方法 应用台盼蓝染色、MTT法、流式细胞仪、甲胎蛋白测定等方法检测ATRA和NaPA两者联合对人肝癌细胞的诱导分化作用. 结果 10μmol·L-1 ATRA，2.5mmol·L-1 NaPA及两者联合作用，可抑制人肝癌细胞株SMMC-7721细胞的生长，使细胞恶性表型向正常转化，其联合作用组7d抑制率达66.5%，并能明显降低甲胎蛋白的分泌量49.2%，使细胞周期G0 /G1 期延长58.7%，S期下降35.8%(P<0.05)，影响DNA合成，最终产生抑制增殖诱导细胞分化的作用. 结论 ATRA，NaPA及两者联合应用均可抑制SMMC-7721细胞生长，诱导细胞分化，ATRA和NaPA联合对人肝癌细胞的诱导分化有协同作用.

    Effects of all┐trans retinoic acid with phenylacete natrium on human HCC cell line

    WU Tao，DOU Ke-Feng，LI Kai-Zong

    Department of Hepatobiliary Surgery，Xijing Hospi-tal，Fourth Military Medical University，Xi'an710033，China

    Keywords:all-trans retinoic acid;phenylacete natrium;hep-atocellular carcinoma;cell differentiation;AFP

    Abstract:AIM To investigate the effects of all-trans retinoic acid in combination with phenylacete natrium on hu-man hepatocellular carcinoma(HCC)cell line SMMC-7721.METHODS HCC cell line was exposed to all-trans retinoic acid and phenylacete natrium separately and both agents in combining manner respectively.The morphologic and func-tional changes of the cell line were observed with benzo blue ......