关键词: α-晶体蛋白;阿斯匹林;布洛芬;酯酶;糖化;氨甲酰化;类固醇

    摘 要:目的 研究阿斯匹林类药物和α-晶体蛋白对酯酶的保护作用. 方法 凝胶色谱分离牛α-晶体蛋白，酯酶分别与糖类、磷酸糖、氰酸钾和激素加阿斯匹林(Asp)、布洛芬(Ibu)、扑热息痛(Para)和α-晶体蛋白温育，采用分光光度法测定酶活性. 结果 糖化、氨甲酰化和类固醇诱导酯酶失活的作用呈浓度依赖性，类固醇失活效应最快.10mmol L-1 Asp可部分保护果糖和类固醇诱导酯酶的失活，尽管Ibu抑制酶活性，但其羧基化(Ibu-1)和羟基化(Ibu-2)代谢物有保护作用.α-晶体蛋白与酯酶摩尔比为1 1时，可完全特异地保护果糖、果糖6-磷酸和泼尼松龙-21-半琥珀酸诱导的酯酶失活;而在1 4时，保护率分别为67.0%，55.0%和38.3%. 结论 Asp和Ibu保护酯酶免于失活的作用机制可能不同;α-晶体蛋白作为分子伴侣可保护酯酶免于失活.

    Aspirin┐like drugs andα┐crystallin protect esterase against inactivation

    YAN Hong HUI Yan-Nian LI Ming-Yong

    1 Department of Ophthalmology，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China，

    2 Department of Ophthalmology，Xijing Hos-pital，

    3 Postgraduate Department，Fourth Military Medical University，Xi'an710033，China

    Keywords:α-crystallin;aspirin;ibuprofen;esterase;glyca-tion;carbamylation;steroid

    Abstract:AIM To investigate the protection effect of as-pirin-like drugs andα-crystallin on inactivation of esterase by glycation.METHODS Bovineα-crystallin was isolated by gel chromatography.Esterase were incubated with sugars ......