克隆分子_____DNA，互补;_克隆分子;

人bit1 cDNA基因的克隆、测序及表达

http://www.100md.com 《第四军医大学学报》 2004年第24期

克隆分子,,DNA，互补;,克隆分子;,序列分析;,基因表达,人bit1cDNA基因的克隆、测序及表达,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Cloning，sequencing and expression of human bit1 cDNA gene

    XING XiaoHong, WU YuanMing, WANG Zhe, WANG WenYong, HUANG GaoSheng

    1Department of Burn, Xijing Hospital,2Department of Pathology, 3Department of Biochemistry, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】AIM： To clone the human bit1 cDNA gene and express it in E. coli. METHODS: Total RNA was extracted from fresh placenta tissue and reverse transcripted to single chain cDNA. By using it as the template, the human bit1 cDNA gene was amplified. The cDNA gene was then cloned into pUC19. After the sequence was verified, the cDNA gene was subcloned into pGEX4T3 for expression. RESULTS: The human bit1 cDNA gene was amplified by using the designed primer and the gene was expressed in E.coli induced by IPTG. The expressed protein accounted for 40.6% of the total amount of protein. CONCLUSION: The human bit1 cDNA gene is amplified and expressed in E.coli, which is important for the study on the function of human bit1 protein. ......

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