Erk信号传导通路调控乙醛刺激的肝星状细胞Ⅰ型胶原分泌及TGFβ1 mRNA的表达
肝纤维化,,肝纤维化;肝星状细胞;细胞外信号调节激酶;乙醛,Erk信号传导通路调控乙醛刺激的肝星状细胞Ⅰ型胶原分泌及TGFβ1mRNA的表达,0引言,1材料和方法,2结果,3讨论,【参考文献】
Effect of extracellularregulated kinase signaling pathways on type Ⅰ collagen synthesis and TGFβ1 mRNA expression stimulated by acetaldehyde in rat hepatic stellate cellsJIANG MingDe, XIE FangWei, OUYANG XueNong,ZENG WeiZheng, GAN XinYu, MA HongDe
1Department of Gastroenterology, General Hospital of Chengdu Military Command, Chengdu 610083, China, 2Department of oncology, Fuzhou General Hospital, Nanjing Military Command Key Department of National Traditional Chinese Medicine & Western Medicine, Fuzhou 350025, China
【Abstract】 AIM: To study the effect of extracellularregulated kinase signaling pathways on typeⅠ collagen synthesis and TGFβ1 expression stimulated by acetaldehyde in rat hepatic stellate cells (HSCs). METHODS: HSCs were isolated from liver of SpragueDawdey rats by in situ perfusion with pronase and collagenase and density gradient centrifugation with metrizamide and cultured. TypeⅠ collagen and transforming growth factor beta 1 mRNA expression in HSCs, stimulated by acetaldehyde predisposed in different doses of PD98059, were detected with ELISA and RTPCR. RESULTS: PD98059 with a concentration of 20 μmol/L suppressed typeⅠ collagen synthesis and TGFβ1 expression in HSCs stimulated by acetaldehyde (P>0.05), and the inhibitory effect of PD98059 at the concentration of 50 μmol/L and 100 μmol/L was significant (P<0.05 or P<0.01)with a dose dependant effect. The TGFβ1 expression and typeⅠ collagen synthesis had a positive correlation (r=0.93). CONCLUSION: Erk signaling pathways in HSCs stimulated by acetaldehyde play an important role in the regulation of Ⅰ collagen synthesis and the expression of TGFβ1 mRNA. The expression of TGFβ1 mRNA inhibited by PD98059 may trigger the decrease of typeⅠ collagen synthesis of HSCs stimulated by acetaldehyde. ......
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