当前位置: 首页 > 期刊 > 《第四军医大学学报》 > 2005年第15期
编号:10948551
实时定量PCR检测小鼠成熟过程中T淋巴细胞sjTRECs水平
http://www.100md.com 《第四军医大学学报》 2005年第15期
实时定量PCR,,实时定量PCR;,信号结合T细胞受体重排删除环;T细胞,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Detection of sjTRECs levels in murine T lymphocytes in process of maturation by realtime quantitative PCR

    ZHANG HuaHua, ZENG YaoYing, ZHAO JingXian, JIANG Xun, HE XianHui, LING Yi

    Insititute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China

    【Abstract】 AIM: To analyze the signal joint T cell receptor rearrangement excision circles(sjTRECs)levels of murine thymocytes and spleen lymphocytes in the process of maturation and to evaluate the amount of nave T cell and thymic function. METHODS: sjTRECs levels were determined by the real time quantitative PCR (SYBR Green). RESULTS: The amount of nave T cells in thymus increased from the age of 1 wk to 6 wk, but at the age of 9 and 12 wk, it began to decrease. The amount of nave T cells increased continually in the spleen from the age of 1 wk to 12 wk. CONCLUSION: In the process of murine maturation, the T cell synthesis function of thymus begins to weaken at 9 wk. The thymic output function is continually enhanced at the maturation process. Realtime PCR is successfully developed and utilized in analyzing the sjTRECs levels and thymic function. ......

您现在查看是摘要页,全文长 10985 字符