大鼠弥漫性脑损伤后Homer蛋白的表达及其意义
弥漫性脑损伤,,Homer;免疫印迹法;弥漫性脑损伤;大鼠,0引言,1材料和方法,2结果,3讨论,【参考文献】
Expression of Homer after diffuse brain injury in rats and its significanceSHI TieJun, FEI Zhou, ZHANG Xiang, HUANG WeiDong, LIANG JingWen, SONG Lei
PLA Institute of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To study the expression and significance of Homer in diffuse brain injury(DBI)of rat in vitro. METHODS: One hundred and five SD rats, weighing(300±25)g, were randomized to normal, shamoperated and DBI groups. The Marmarou rats were applied to DBI group and the shamoperated group was treated as DBI group except for “free fall” attack. The rats main brain areas and nuclei were taken to detect for Homer by immunoblotting at 0.5, 1, 6, 12, 24, 72 and 168 h after injury respectively. RESULTS: Homer1a was positive in DBI group from 0.5 h to 168 h after injury but no Homer1a was observed in normal group and shamoperated group. Homer1b/c, Homer2a/b and Homer3 were present in the normal, shamoperated and DBI groups. CONCLUSION: There is a significant change in Homer1a expression before and after injury, but there is no significant change in Homer1b/c, Homer2a/b and Homer3 levels, which indicates that Homer1a is involved in the neuronal injury and may protect neurons from insults. ......
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