SARS病毒__(期刊论文)

实时荧光RTPCR对SARS患者血清中病毒含量的定量检测

http://www.100md.com 《第四军医大学学报》 2005年第23期

SARS病毒,,SARS病毒；逆转录聚合酶链反应；定量分析；血清,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Realtime fluorescent RTPCR quantitative detection of SARS CoV in serum of infected patients

    LI ZhenYong， SHAO DaXiao， FENG YanMing，LI ZhiTao， JING JianZhou2，WANG YunLong， QU LingBo， ZHAO YuFen

    1Key Laboratory of Chemobiology, Faculty of Chemistry, Zhengzhou University, Zhengzhou 450052, China, 2Henan Provincial Research Centre of Biotechnology, Zhengzhou 450056, China

    【Abstract】 AIM: To detect SARSCoV in various clinical specimens such as sputum, gargling liquid, throat swab and stool and explore the association between the disease course and the SARSCoV content. METHODS: We quantitatively detected 156 serum specimens from SARS patients by real time RTPCR. The viral load in SARSCoV positive serum was analyzed using the coronavirus deactivated with 9.4×109 copies/L. Robert Koch Institute as standard preparation and viruslike particles as positive controls. RESULTS: The sensitivity of the detected agent was 1×105 copies/L. Eightyone of the 156 cases (51.9%) tested were positive with the viral load ranging from 1×105-1×107 copies/L. The viral load in serum samples was generally low as compared with data reported for other specimens. CONCLUSION: There is no significant relationship between the low viral load and the disease course. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/03/12/94/84/95.htm

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