Effects of Danshen injection on ICAMª²1 expression in hepatocellular carcinoma with malignant biliary obstruction

    XIA Rongª²Long, ZHENG Lanª²Dong, LIU Qingª²Guang, PAN Chengª²En

    1Department of Hepatobiliary Surgery, First Hospital, Henan University, Kaifeng 475001, China, 2Department of Hepatobiliary Surgery, First Hospital, Xi¬ðan Jiaotong University, Xi¬ðan 750061, China

    ¡¾Abstract¡¿ AIM: To establish a malignant biliary obstruction model of SD rats and to study the effects of Danshen injection on the diameter of tumor, inhibitory rates and metastasic rates of hepatocellular carcinoma and on the expression of intercellular adhesion moleculeª²1 (ICAMª²1) in carcinoma and periª²carcinoma tissues, adjacent lobes (leftª²internal lobe) as well as lung tissues. METHODS: Malignant biliary obstruction model of SD rats was made by vaccination of transplanted tumor in liver near the porta hepatis with walkerª²256 hepatocarcinoma cell line infiltrating and metastasizing to proximal common bile duct. The SD rats were divided into four groups and each group were treated respectively by 0.9% NS (n=24), by inosine+Vitamin C (n=40), by Danshen (n=40) and by 5ª²FU (n=40). Observations were made on the diameter of the tumors, the inhibitory rates, the metastasic rates of hepatocellular carcinoma and the expression of ICAMª²1 in carcinoma and periª²carcinoma tissues, adjacent lobes (leftª²internal lobe) as well as lung tissues. Statistical analysis was conducted. RESULTS: The diameters of tumors were reduced and both the tumor and metastasis inhibitory rates of hepatocellular carcinoma were enhanced after the treatment of Danshen injection, compared with those in rats treated by 0.9% NS and inosine combined with Vitamin C (P<0.01). The inhibitory rates(41.7%) and liver¬ðs and lung¬ðs metastasic rates (59.7% and 81.1%) of hepatocellular carcinoma in rats treated by Danshen injection were similar to those in rats treated by 5ª²FU (P>0.05) except lung¬ðs metastasic rates (P<0.01). The expression of ICAMª²1 in carcinoma, periª²carcinoma adjacent lobe tissues as well as lung tissues was significantly reduced after treatment of Danshen injection compared with that in rats treated by 0.9% NS and inosine combined with Vitamine C (P<0.01) and in rats treated by 5ª²FU (P<0.05). CONCLUSION: In SD rat model of malignant biliary obstruction, Danshen, can inhibit the growth of hepatocarcinoma through reducing proliferation of hepatocarcinoma cells, increasing the reversion of hepatocarcinoma cells and decreasing the expression of ICAMª²1 in carcinoma, periª²carcinoma adjacent lobe tissues as well as lung tissues. ......