Biocompatibility between two different biomaterials and cultured hippocampusª²derived neural stem cells in vitro

    GUO Baoª²Feng£¬ DONG Mingª²Min, GUO Zhangª²Sheng

    1Department of Otolaryngology, First Affiliated Hospital, Zhengzhou University, 2Department of Otolaryngology, Hospital of Henan Provincial Crops, Chinese People¬ðs Armed Police Force, 3Department of Anatamy, School of Basic Medicine, Zhengzhou University, Zhengzhou 450052, China

    ¡¾Abstract¡¿ AIM: To study the biocompatibility between two different biomaterials (collagen protein sponge and glutin sponge) and cultured hippocampusª²derived neural stem cells in vitro and to identify whether the materials could be used as scaffold biomaterials in peripheral neural tissue engineering. METHODS: Hippocampusª²derived neural stem cells of guinea pigs were cultured and respectively combined with each of the two biomaterials in vitro. The number of cells was counted and the histological changes were observed with HE staining and scanning electron microscope after 7 d and the adhesion rates were detected. RESULTS: Hippocampusª²derived neural stem cells attached to both biomaterials and normally grew. The adhesion rates were 31.17% and 14.87% respectively. The total numbers of the cells in the experimental and control groups were similar and there was no statistical difference. CONCLUSION: Both biomaterials have a good biocompatibility with hippocampusª²derived neural stem cells of guinea pigs and can be used safely as scaffold materials in peripheral neural tissue engineering. ......