抗纤I号对肝星状细胞增殖及相关基因表达的影响
肝星状细胞,,抗纤I号;转化生长因子β1;,肝星状细胞;血小板衍生生长因子,0引言,1材料和方法,2结果,3讨论,【参考文献】
Effects of antifibrosis herbal compound on proliferation of hepatic stellate cells and expression of related genesGAO QingHua, LIU DianWu, LIU YaXin, JIANG ChangWen, LI Qing
1Department of Epidemiology, 3Department of Cell Biology, Hebei Medical University, Shijiazhuang 050017, China, 2Tangshan Professional and Technological College, Tangshan 063000, China
【Abstract】 AIM: To investigate the effects of antifibrosis herbal compound on activated hepatic stellate cells and to explore its molecular mechanism in the treatment and prevention of hepatic fibrosis. METHODS: The transforming growth factorβ1 (TGFβ1) and platelet derived growth factor (PDGF) mRNA were detected by RTPCR method. The protein of TGFβ1 and PDGF was determined by Western Blot analysis. The cell growth curve was also observed with MTT assay. RESULTS: After treated with 100 mL/L drug serum of antifibrosis herbal compound and 100 mL/L colchicine serum for 24 h, the mRNA and protein expression of TGFβ1 and hepatic stellate cells PDGF decreased sharply compared with those in control group (P<0.05). The mRNA and protein expression of TGFβ1 and PDGF in antifibrosis herbal compound group was lower than that in colchicine group (P<0.05). MTT assay revealed that the proliferation of the activated hepatic stellate cells in colchicine group and antifibrosis herbal compound group reduced significantly compared with those in control group (P<0.05). CONCLUSION: Antifibrosis herbal compound can treat hepatic fibrosis by inhibiting the expression of TGFβ1 and PDGF of the activated hepatic stellate cells. ......
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