肝星状细胞_____抗纤I号；转化生长因子β1；

抗纤I号对肝星状细胞增殖及相关基因表达的影响

http://www.100md.com 《第四军医大学学报》 2005年第22期

肝星状细胞,,抗纤I号；转化生长因子β1；,肝星状细胞；血小板衍生生长因子,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effects of antifibrosis herbal compound on proliferation of hepatic stellate cells and expression of related genes

    GAO QingHua, LIU DianWu, LIU YaXin, JIANG ChangWen, LI Qing

    1Department of Epidemiology, 3Department of Cell Biology, Hebei Medical University, Shijiazhuang 050017, China, 2Tangshan Professional and Technological College, Tangshan 063000, China

    【Abstract】 AIM: To investigate the effects of antifibrosis herbal compound on activated hepatic stellate cells and to explore its molecular mechanism in the treatment and prevention of hepatic fibrosis. METHODS: The transforming growth factorβ1 (TGFβ1) and platelet derived growth factor (PDGF) mRNA were detected by RTPCR method. The protein of TGFβ1 and PDGF was determined by Western Blot analysis. The cell growth curve was also observed with MTT assay. RESULTS: After treated with 100 mL/L drug serum of antifibrosis herbal compound and 100 mL/L colchicine serum for 24 h, the mRNA and protein expression of TGFβ1 and hepatic stellate cells PDGF decreased sharply compared with those in control group (P<0.05). The mRNA and protein expression of TGFβ1 and PDGF in antifibrosis herbal compound group was lower than that in colchicine group (P<0.05). MTT assay revealed that the proliferation of the activated hepatic stellate cells in colchicine group and antifibrosis herbal compound group reduced significantly compared with those in control group (P<0.05). CONCLUSION: Antifibrosis herbal compound can treat hepatic fibrosis by inhibiting the expression of TGFβ1 and PDGF of the activated hepatic stellate cells. ......

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