绿色荧光蛋白__(期刊论文)

pTracer/mIFNγ基因修饰对小鼠胚胎干细胞细胞生物学特性的影响

http://www.100md.com 《第四军医大学学报》 2005年第17期

绿色荧光蛋白,,干扰素γ；绿色荧光蛋白；胚胎干细胞；生物学特性,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Study on biological properties of mouse embryonic stem cells modified by pTracer/mIFNγ

    ZHANG XiaoZhi, CHEN Wei, LI Xu, XU HaiWei

    1Department of Radiotherapy, 2Medical Experiment Center, First Hospital, Xian Jiaotong University, Xian 710061, 3Department of Physiology, Third Military Medical University, Chongqing 430038, China

    【Abstract】 AIM: To study the characteristics of embryonic stem (ES) cells transfected with pTracer/mIFNγ. METHODS: The gene pTracer/mIFNγ including IFNγand green fluorescent protein (GFP) was transfected to obtain ES/ pTracer/mIFNγ cells by electroporation. RTPCR and ELISA were used to detect IFNγ expression at transcription and protein level respectively and green fluorescence was detected with fluorescence microscope to prove the GFP expression. Colony formation rates for ES/pTracer/mIFNγ cells and ES cell were conducted respectively. ES/pTracer/mIFNγ cells were induced to differentiate into neural precursor cells (NPCs) in serumfree selective medium and nestin immunoreactive was identified by immunocytochemistry. RESULTS: Green fluorescence was observed in ES/pTracer/mIFNγ cells and IFNγ concentration was (44.25±12.23)μg/L 72 h after gene transfection. ES/pTracer/mIFNγ cells were positive to alkaline phosphatase. The cell colony formation rates of ES cells and ES/pTracer/mIFNγ cells were 73.66% and 70.89%, respectively (P>0.05). ES/pTracer/mIFNγ cells differentiated into NPCs, which had potency to differentiate into all kinds of neural cells. CONCLUSION: The cells modified with IFNγgene may not influence the proliferation, differentiation and the pluripotentiality of ES cells. ......

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