基因转染_____Caspase3基因；

GFP共表达的重构型Caspase3基因对Hep2细胞的作用

http://www.100md.com 《第四军医大学学报》 2005年第21期

基因转染,,Caspase3基因；,凋亡；,基因转染,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effects of recombinant Caspase3 on human laryngocarcinoma cell line Hep2 through coexpression with GFP

    YU XiaoLin, LIU Hong, CHEN WanPing, CHENG PengYuan

    Outpatient Department, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To investigate the apoptosis induction effect of the expression of recombinant Caspase3 on laryngocarcinoma cell line Hep2. METHODS: Recombinant Caspase3 gene was subcloned into the GFP reporting vector pEGFPC1 to generate the expressing vector pEGFPCaspase3 by DNA recombinant technique. Human laryngocarcinoma cell line Hep2 was transfected with Caspase3 gene by Lipofectamine 2000 and the expression of recombinant Caspase3 was observed by RTPCR. The cell morphological changes were observed under fluorescent and electronic microscope and the cell survival rate after transfection was assayed by MTT method. RESULTS: Recombinant Caspase3 gene was stably expressed in Hep2. Recombinant Caspase3 induced obvious apoptosis in vitro. The MTT results suggested that the cell curve declined after transfection with Caspase3. CONCLUSION: Recombinant Caspase3 can inhibit the growth of the laryngocarcinoma cell line Hep2 and effectively induce it to apoptosis. ......

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