DNA polymerase ¦Â gene mutation in human nasopharyngeal cancer and its relationship with EBV infection

    ZHENG Hong, LI Mingª²Shan, ZHAO Guoª²Qiang, DONG Ziª²Ming, SONG Baoª²Yi

    1Department of Stomatology, Medical College of Henan University, Kaifeng 475001, China, 2Department of Pathophysiology, Basic Medical College of Zhengzhou University, Zhengzhou 450052£¬China, 3Department of Ophthalmology & Otorhinolaryngology, PLA 155th Hospital, Kaifeng 475003£¬China

    ¡¾Abstract¡¿ AIM: To study DNA polymerase ¦Â (pol¦Â) gene mutation in human nasopharyngeal cancer and its relationship with EBV. METHODS: Twentyª²six cases of nasopharyngeal carcinoma tissues and corresponding normal epithelial tissues were collected to study DNA pol¦Â gene mutation with PTª²PCR, PCRª²SSCP and DNA sequence analyzing techniques. DNA was extracted and amplified with EBV primers and the results were observed and analyzed. RESULTS: pol¦Â gene mutation was detected by SSCP in 8 of the 26 nasopharyngeal cancer tissues (with mutation rate 30.8%) and there was no mutation in juxtacancerous tissues. Many types of mutation were found by DNA sequencing. EBVª²DNA positive rate was 92.3%. The results of experiment indicated that the cases of pol¦Âª²SSCP abnormal were accompanied with EBV infection. CONCLUSION: There is pol¦Â alternation in human nasopharyngeal carcinoma, which leads to the sequence alternation in amino acid and spatial structure change. These mutations and EBV infection may play an important role in tumor genesis. ......