Inhibition of MGC803 growth in nude mice by human dendritic cells transfected with pcDNA3ª²hCEA

    MA Junª²Fen, HUANG Youª²Tian, ZHAO Mingª²Yao, YANG Hongª²Yan, ZHENG Zhiª²Min, DONG Ziª²Ming

    Department of Pathophysiology, Basic Medical College, Zhengzhou University, Zhengzhou 450052, China

    ¡¾Abstract¡¿ AIM: To investigate the inhibition of dendritic cells (DCs) (pcDNA3ª²hCEA) inoculation on MGC803 in nude mice. METHODS: DCs were generated from human fresh peripheral blood in the presence of rhGMª²CSF and rhILª²4 and DC vaccine was prepared by transfection with pcDNA3ª²hCEA using lipofectine. CEA mRNA expressing in DCs (pcDNA3ª²hCEA ) was confirmed by RTª²PCR and specific cytotoxic T lymphocytes (CTL) was induced in vitro. The inhibition of the DC (pcDNA3ª²hCEA) inoculating on MGC803 in nude mice was observed. RESULTS: CEA mRNA of DCs (pcDNA3ª²hCEA ) could be detected at 588 bp with a pair of specific primers by RTª²PCR and the specific CTL (%) at effectª²target rate 10¡Ã1, 20¡Ã1, 40¡Ã1 were respectively 19.4¡À3.8, 24.7¡À3.7, 38.1¡À5.4. DCs (pcDNA3ª²hCEA) effectively inhibited the growth of MGC803. The median of tumor genesis time in the experiment group was longer than that of control (10 d vs 6 d, n=5) and the growth speed of tumor inoculating on DCs (pcDNA3ª²hCEA) in nude mice was slower than that in control group. The size of tumor inoculating on DCs (pcDNA3ª²hCEA) in nude mice was less than that in control group [(0.24¡À0.10) cm3 vs (1.36¡À0.42) cm3, n=5, P<0.05]. CONCLUSION: The human dendritic cells transfected with pcDNA3ª²hCEA can inhibit MGC803 cells in nude mice. ......