DNA聚合酶β_____DNA聚合酶β；基因突变；胃肿瘤

胃癌组织中DNA聚合酶β基因突变特点分析

http://www.100md.com 《第四军医大学学报》 2005年第21期

DNA聚合酶β,,DNA聚合酶β；基因突变；胃肿瘤,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Mutation characteristics of DNA polymerase beta gene in human gastric carcinoma tissue

    ZHAO Jie, DONG ZiMing, MAO HaiZhou, WANG Xuan, ZHAO GuoQiang

    1Department of Gastroenterology, PLA 155 Central Hospital, Jinan Military Area Command, Kaifeng 475003, China, 2Department of Pathophysioloy, College of Basic Mediine, Zhengzhou University, Zhengzhou 450052, China

    【Abstract】 AIM: To detect the mutation characteristics of DNA polymerase beta gene (polβ gene) in human gastric carcinoma. METHODS: Total RNA was extracted from 32 gastric cancer samples and 32 tissue samples adjacent to tumors. The RTPCR was carried out and the PCR product was cloned and sequenced with Sangers method subsequently. RESULTS: Three from the seven abnormal SSCP samples were sequenced and the mutation sites were: site 196 A→G, site 268 A→G and site 790 A→T. No mutation was found by sequencing in a normal PCRSSCP sample of the tissues adjacent to the tumor. Omiga analysis showed that the three point mutations led to changes of amino acid: site 196 A→G of nucleic acid resulted in site 28 Asn→Ser of amino acid, site 268 A→G of nucleic acid resulted in site 52 Lys→Arg of amino acid, and site 790 A→T of nucleic acid resulted in site 226 Asp→Val of amino acid. Choufasman method of Dnasis showed that site 196 A→G mutation caused great changes in the secondary structure of polβenzyme. CONCLUSION: The polβgene mutation rate is up to 21.9%. The three point mutations observed in this study all lead to the alternation of amino acid. Site 196 A→G mutation can cause great changes in the secondary structure of polβ enzyme. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/03/12/94/99/07.htm

您现在查看是摘要页，全文长 6922 字符。