Effect of cigarette smoke extract on proliferation and cycle of human airway epithelial cell and its FIP200 expression

    XU Li£¬ZHANG Zhenª²Xiang£¬XU Yongª²Jian

    Department of Respiratory Diseases£¬ Tongji Hospital Affiliated to Tongji Medical College£¬ Huazhong University of Science and Technology£¬Wuhan 430030£¬China

    ¡¾Abstract¡¿ AIM: To study the effect of cigarette smoke extract (CSE ) on the proliferation and cycle of human airway epithelial cell and its mechanism. METHODS: After airway epithelial cells were treated with CSE, the proliferation was measured with MTT and the distribution of different cell cycles was detected with flow cytometry. FAKª²family interacting protein of Mr 200 000 (FIP200) mRNA expression level, FAKª²family interacting protein of Mr 200 000(FIP200)expression level and the association of FIP200 with focal adhesion kinase(FAK)were analyzed respectively by reverse transcriptionª²polymerase chain reaction(RTª²PCR), Western blot and immunoprecipitation. RESULTS: CSE inhibited the proliferation of human airway epithelial cells and arrested the epithelial cells in G1 phase of cell cycle. The number of epithelial cells in S and G2 phase dramatically decreased (P<0.01). CSE increased the expression level of FIP200 mRNA and FIP200 and the association of FIP200 with FAK(P<0.01). The effects of CSE on airway epithelial cells were all in a doseª²andª²time dependent manner. The expression of FIP200 mRNA and FIP200 and the association of FIP200 with FAK were positively correlated to the increased number of epithelial cells in G1 phase(P<0.01) and negatively correlated to the proliferation and the number of epithelial cells in S and G2 phase(P<0.01). CONCLUSION: CSE can inhibit the proliferation of human epithelial cells and the increased expression of FIP200 may be one of the factors responsible for it. ......