Influences of arginine vasopressin and its receptors on iNOSª²NO system activity in cardiac fibroblasts

    FAN Yanª²Hong, ZHAO Lianª²You, WANG Haiª²Chang

    1Department of Cardiology, Xijing Hospital, Xi¬ðan 710033, China, 2Department of Cardiology, Tangdu Hospital, Xi¬ðan 710038, China, Fourth Military Medical University

    ¡¾Abstract¡¿ AIM: To explore the influences of arginine vasoª²pressin (AVP) and its receptors on inducible nitric oxide synthaseª²nitric oxide (iNOSª²NO) system activity in cardiac fibroblasts (CFs). METHODS: After CFs were isolated and cultured, nitric acid reductase method, spectrophotometry and RTª²PCR were used to detect the changes of iNOSª²NO system activity induced by AVP and its receptors. RESULTS: AVP significantly enhanced CFs iNOSª²NO system activity. V1 receptor antagonist inhibited iNOSª²NO system activity in a concentrationª²dependent manner and 1¡Á10-7 mol/L V1 receptor antagonist decreased iNOSª²NO system activity to the control level. V1 receptor antagonist and V1+V2 receptor antagonist had the same effects on iNOSª²NO system activity. CONCLUSION: V1 receptor antagonist mediated iNOSª²NO system activity increases in AVPª²induced CFs. V1 receptor antagonist may be a new target in the prevention and reversion of cardiac remodeling. ......