人眼小梁组织及体外培养的人眼小梁细胞SPARC mRNA及其蛋白表达
人眼,,小梁组织;小梁细胞;酸性富含胱氨酸分泌型蛋白;,细胞外基质;原发性开角型青光眼,人眼小梁组织及体外培养的人眼小梁细胞SPARCmRNA及其蛋白表达,0引言,1材料和方法,2结果,3讨论
Expression of SPARC mRNA and SPARC protein in human trabecular meshwork and cultured human trabecular meshwork cells in vitroLIU SiWei, XIAO Ying, ZHANG DeXiu
Department of Ophthalmology,First Affiliated Hospital, Medical College, Xian Jiaotong University, Xian 710061, China
【Abstract】 AIM: To investigate whether secreted protein acidic rich in cysteine(SPARC) and SPARC mRNA are expressed in human trabecular meshwork(TM) and cultured human trabecular meshwork cells(TMCs) and to explore the roles of SPARC protein in the pathogenesis of primary openangle glaucoma (POAG). METHODS: RTPCR and WesternBlot were used to detect SPARC mRNA and SPARC protein and immunofluorescence staining of cultured TMCs was performed by antiSPARC antibody. RESULTS: RTPCR of TM and TMCs using SPARC specific primers of the RNA displayed the presence of the predicted band of approximately 300 bp. WesternBlot showed a protein band of 43 Ku. CONCLUSION: Human TM and TMCs can express SPARC mRNA and its correlative protein, which influences the accumulation and degradation of extracellular matrix. ......
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