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人眼小梁组织及体外培养的人眼小梁细胞SPARC mRNA及其蛋白表达
http://www.100md.com 《第四军医大学学报》 2006年第3期
人眼,,小梁组织;小梁细胞;酸性富含胱氨酸分泌型蛋白;,细胞外基质;原发性开角型青光眼,人眼小梁组织及体外培养的人眼小梁细胞SPARCmRNA及其蛋白表达,0引言,1材料和方法,2结果,3讨论
     Expression of SPARC mRNA and SPARC protein in human trabecular meshwork and cultured human trabecular meshwork cells in vitro

    LIU SiWei, XIAO Ying, ZHANG DeXiu

    Department of Ophthalmology,First Affiliated Hospital, Medical College, Xian Jiaotong University, Xian 710061, China

    【Abstract】 AIM: To investigate whether secreted protein acidic rich in cysteine(SPARC) and SPARC mRNA are expressed in human trabecular meshwork(TM) and cultured human trabecular meshwork cells(TMCs) and to explore the roles of SPARC protein in the pathogenesis of primary openangle glaucoma (POAG). METHODS: RTPCR and WesternBlot were used to detect SPARC mRNA and SPARC protein and immunofluorescence staining of cultured TMCs was performed by antiSPARC antibody. RESULTS: RTPCR of TM and TMCs using SPARC specific primers of the RNA displayed the presence of the predicted band of approximately 300 bp. WesternBlot showed a protein band of 43 Ku. CONCLUSION: Human TM and TMCs can express SPARC mRNA and its correlative protein, which influences the accumulation and degradation of extracellular matrix. ......

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