细胞_____PC12细胞；缺糖损伤；凋亡

PC12细胞缺糖损伤的分子生物学特征

http://www.100md.com 《滨州医学院学报》 2006年第1期

细胞,,PC12细胞；缺糖损伤；凋亡,1材料与方法,2结果,3讨论,参考文献

     【摘要】目的在体外模拟能量代谢障碍研究PC12细胞缺糖损伤的分子生物学特征。方法电镜观测形态改变，MTT检测功能异常，RTPCR测定Bcl2在RNA水平上的表达。结果电镜观察到细胞有凋亡现象；MTT显示细胞活力降低；RTPCR结果表明Bcl2在6~16 h表达上调。结论缺糖损伤的PC12细胞既有形态的变化也有功能上的变化，并同时伴随着凋亡和坏死两种死亡现象。

    【关键词】 PC12细胞；缺糖损伤；凋亡

    Cellular and molecular alterations of PC12 cells deprived of glucose

    SONG Xiaodong JIANG Shaofeng NIU Xinhua et al

    Department of Biology,Binzhou Medical College,Binzhou 256603

    【Abstract】 Objective By using glucose deprivation (an insult relevant to the brain ischemia) media,PC12 cell strain was evaluated.Methods By cellular and molecular biological techniques including electron microscopy,MTT,and RTPCR to meaure and observe.Results Electron microscopy reveals the treated PC12 cells underwent apoptosis;MTT result shows the treated PC12 cells viabilities induced;Bcl2 RNA levels expressed to peak levels 616 hours after glucose deprivation treatment and then declined gradually.Conclusion The PC12 cells deprived of glucose underwent the morphological and functional alterations including apoptosis and necrosis. ......

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