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氯沙坦通过减少细胞凋亡减轻心肌肥大大鼠的骨骼肌萎缩
http://www.100md.com 《第四军医大学学报》 2006年第3期
氯沙坦,,细胞凋亡;心肌肥大;骨骼肌;氯沙坦,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Losartan decreases skeletal muscle atrophy in rats with myocardial hypertrophy through reducing cell apoptosis

    FU ChunJing,HUANG YouTian, GUO LongHui, SHEN Qi, TANG Ning

    1Department of Pathophysiology, Medical College, 2Department of Cardiovascular Surgery, Second Affiliated Hospital, 3Department of Computer, Secondary Hygienic School, Zhengzhou University, Zhengzhou 450052, China

    【Abstract】 AIM: To explore the role of cell apoptosis in skeletal muscle atrophy in rats with myocardial hypertrophy and the effect of losartan on it. METHODS: Fortytwo Sprague Dawley rats were randomly divided into 3 groups: group I (n=14, normal control group), group II (n=14, myocardial hypertrophy group) and group III (n=14, losartan group). Monocrotaline (30 mg/kg) was injected transperitoneally in group II and group III to induce myocardial hypertrophy. After 2 weeks, group III was treated with losartan [10 mg /(kg·d)] by gavage. After 2 additional weeks, the rats were killed. Gastrocnemius mass /body mass ratio served as the degree of muscle atrophy. Apoptotic cells in gastrocnemius were stained in situ by using TUNEL. Gastrocnemius Bcl2 and Bax protein were quantified by immunhistochemistry and Western Blot. Bax /bcl2 served as the degree of apoptosis. RESULTS: The ratio of apoptosis was higher in group II than that in group I [(147.6±32.6)‰ vs (4.1±1.8)‰, P<0.05]. The ratio of apoptosis was lower in group III than that in group II [(46.2±12.3)‰ vs (147.6±32.6)‰, P<0.05]. The atrophy of skeletal muscle in group II was more serious that in group I(P<0.05), but that in group III was less serious than that in group II. The expression of Bcl2 in group II was significantly lower than that in group I(P<0.05), but the expression of Bax in group II was significantly higher than that in group I(P<0.05). The expression of Bcl2 in group III was higher than that in group II(P<0.05)but the expression of Bax was lower that in group II(P<0.05). The ratio of Bax/Bcl2 in group II was higher than that in group III. CONCLUSION: Cell apoptosis plays an important role in skeletal muscle atrophy in rats with myocardial hypertrophy and may be one of the factors causing skeletal muscle atrophy. Losarton can decrease skeletal muscle cell apoptosis and atrophy in myocardial hypertrophy rat through regulating the ratio of Bax / Bcl2. ......

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