关键词: 安定;凋亡;坏死;脑缺血;脑梗塞

    摘 要:目的 研究安定对光化学诱发脑梗死后细胞死亡的保护作用. 方法 使用光化学诱发的大鼠局灶性脑缺血模型.采用原位末端标记技术(TUNEL)检测凋亡细胞.大鼠脑梗死术后存活3，6，12，24，48和72h，分别观察梗死灶坏死中心面积和凋亡细胞数目.术前24h开始腹腔注射安定(10mg·kg-1 ·8h-1 )，直到处死动物. 结果 TUNEL阳性细胞位于坏死中心与正常皮层之间的半影区.TUNEL阳性细胞的均数随着梗死后时间的延长逐渐增多，24h达到高峰.在24h时间点，安定治疗组梗死坏死中心的平均最大面积和TUNEL阳性细胞均数与对照组相比，明显减少(P<0.001). 结论 安定可明显减少成年大鼠局灶性脑梗死后的细胞坏死和凋亡.该结果可为临床使用安定治疗缺血性脑血管病提供理论依据.

    Protective effects of diazepam against nervous cell death in adult rats with cerebral ischemia

    WU Zhong-Liang，LIU Fei，YOU Si-Wei，JIAO Xi-Ying，JU Gong

    Institute of Neurosciences of Chinese PLA，Fourth Military Medical University，Xi'an710033，China Keywords:diazepam;apoptosis;necrosis;cerebral ischemia;cerebral infarction

    Abstract:AIM To study the protective effects of diazepam against nervous cell death after photochemically induced cere-bral ischemia.METHODS Focal cerebral ischemia induced by photothrombosis in adult rats was used as a model.Apop-tosis was detected by terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling(TUNEL).The areas of necrotic cores and the numbers of apoptotic cells were studied quantitatively in the infarcted area at3 ......