关键词: 肝炎病毒，乙型;C型肝炎样病毒属;细胞系

    摘 要:目的 建立融合表达HBV表面抗原与HCV核心蛋白的体外培养细胞系，使之作为细胞杀伤实验的靶细胞. 方法 将含HBV S和HCV C融合基因真核表达质粒CSpcD-NA3.1用电穿孔法转染p815细胞，G418筛选后，再用有限稀释法进行单细胞克隆筛选.RT-PCR与免疫荧光法分别检测基因转录与蛋白表达. 结果 HBV S基因与HCV C基因的RT-PCR检测均为阳性，HBV表面抗原与HCV核心蛋白的免疫荧光检测均为阳性. 结论 成功获得融合表达HBV表面抗原与HCV核心蛋白的细胞系.

    Establishment of a cell line expressing HBsAg/HCcAg fusion protein

    YIN Wen XU Zhi-Kai XUE Xiao-Ping MA Yue-Yun Liu Wei-Dong FU Li LU Xin

    1 Department of Microbiology，Faculty of Preclinical Medicine，

    2 Research Laboratory of Clinical Molecu-lar Biology，Xijing Hospital，Fourth Military Medi-cal University，Xi'an710033，China

    Keywords:hepatitis B virus;hepatitis C-like viruses;cell line

    Abstract:AIM To establish the stable cell line expresssing HBV surface antigen and HCV core protein and to use it as the target cell for cytotoxic T-cell response.METHODS The p815cell was transfected by the chimeric plasmid CSpcDNA3.1including HBV S gene and HCV C gene.Clones which expressed HBV surface antigen(HBsAg)and HCV core protein(HCcAg)were selected with G418and lim-ited dilution.HBV S gene and HCV C gene were detected by RT-PCR ......