关键词: 哮喘;嗜酸粒细胞;嗜酸粒细胞趋化因子;嗜酸粒细胞阳离子蛋白

    摘 要:目的 探讨哮喘发病中嗜酸粒细胞趋化因子(Eotax-in)基因表达与嗜酸粒细胞(Eos)活化的关系及糖皮质激素的作用. 方法 将豚鼠30只随机分为哮喘组、地塞米松治疗组及正常对照组.卵蛋白致敏及诱发哮喘;制备豚鼠肺组织病理标本，HE染色;免疫组织化学技术观察Eos阳离子蛋白(ECP)在哮喘气道粘膜中的表达;原位分子杂交方法观察肺组织中Eotaxin mRNA的表达;将二者行相关性分析. 结果 与地塞米松治疗组、正常对照组相比较，哮喘组豚鼠肺组织中ECP细胞(172±44)·mm-2 与eotaxin mRNA(196±57)·mm-2 表达明显增强(P<0.001)，Eos活化增多;且二者呈正相关性(r=0.7447，P=0.0135);糖皮质激素对Eotaxin mRNA(20±14)·mm-2 ，ECP(26±19)·mm-2 表达有显著的抑制作用. 结论 哮喘豚鼠肺组织中Eotaxin表达增强，Eos活化增多.糖皮质激素能抑制Eotaxin表达及Eos活化.

    Correlation between eotaxin gene expression and eosinophils activation in lung tissue of asthmatic guinea pigs

    CHEN Xin YU Hua-Peng QI Hao-Wen

    1 Department of Respiratory Diseases，Zhujiang Hos-pital，First Military Medical University，Guangzhou510282，China，

    2 Department of Respiratory Dis-eases，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:asthma;eosinophil;eotaxin;eosinophil cation protein

    Abstract:AIM To study the relationship between gene ex-pression of eotaxin and eosinophils activating in lung tissue of asthmatic guinea pigs，and to study the essential function of corticosteroid in them.METHODS Thirty guinea pigs were divided into three groups(asthma ......