关键词: 输血传播，病毒;肝炎，非甲～戊型;核酸，序列

    摘 要:目的 了解山东地区输血传播病毒(TTV)分离株感染状况及基因变异的情况. 方法 应用逆转录—聚合酶链反应法(RT-PCR)检测山东地区26例非甲～戊型肝炎和12例肝细胞癌患者血清中的TTV DNA，对阳性扩增的产物进行测序，并分析其基因变异情况. 结果 26例非甲～戊型肝炎患者检出TTV DNA阳性者11例(42%).对其中2例(TTVSD4，TTVSD5)进行PCR产物直接测序，并与日本株(AB008394)相比较，其核苷酸序列同源性分别为99.9%和100%.而12例肝细胞癌患者中TTVDNA阳性3例(25%)，对其中1例(TTVSD6)测序，与日本株(AB008394)相比较，其核苷酸序列同源性为99%;TTVSD4、TTVSD5和TTVSD6之间的核苷酸同源性均为99%. 结论 山东地区非甲～戊型肝炎和肝细胞癌患者中TTV感染率较高，测序结果表明其与日本株(AB008394)有较高的同源性.

    Partial gene of transfusion transmitted virus(TTV)detecting and sequencing in Shandong

    YU Jian-Guo SHANG Qing-Hua REN Hao XIAO De-Ming YI Yan-Ming BAI Wei QI Zhong-Tian ZHANG Guang-Shu

    1 Institute of Liver Disease，Jinan Military Region，Department of Infectious Diseases，88Hospital of Chinese PLA，Taian271000，China，

    2 Department of Microbiology，Second Military Medical Universi-ty，Shanghai200433，China.

    3 Department of Infec-tious Diseases，Taian City Central Hospital，

    4 Affiliated Hospital of Taishan Medical College，Ta-ian271000，China

    Keywords:transfusion transmitted virus;nonA-E hepatitis;Nucleotide sequence

    Abstract:AIM To investigate the prevalence of TTV infec-tion and genetic variation of TTV isolate in Shandong Province.METHODS TTV DNA were amplified and de-tected by RT-PCR methods from sera of patients with nonA-E hepatitis(n=26)and hepatocellular carcinoma(n=12).The positive PCR products were sequenced and analyzed for genetic variation.RESULTS TTV DNA were detectable in11of26patients(42.3%)with nonA-E hepatitis.Two of them(TTVSD4 ......