关键词: 氯离子通道阻断剂;气管条;豚鼠;5-羟色胺;钙离子

    摘 要:目的 研究氯离子通道阻断剂对豚鼠离体气管条收缩反应的影响及其作用机制. 方法 制备离体孵育的豚鼠气管条，分别在浴液中加入药物，测定KCl和5-羟色胺(5-HT)收缩气管条的作用以及氯离子通道阻断剂和维拉帕米(Ver)对KCl和5-HT作用的影响. 结果 两种氯离子通道阻断剂氮氟灭酸(NFA)和5-硝基-2-3苯酚丙胺苯甲酸盐(NPPB)均能使KCl或5-HT引起的气管条收缩显著抑制，解痉百分率分别为100%和30%，Ver的抑制效应位于NFA与NPPB之间，NFA，NPPB和Ver对细胞内Ca2+ 释放和细胞外Ca2+ 内流引起的收缩均有抑制作用. 结论 氯离子通道阻断剂NPPB和NFA可抑制KCl和5-HT引起的气管条收缩，其机制可能是氯离子通道阻断剂影响细胞钙运动.

    Effects of chloride┐channel blockers on the contrac┐tion of isolated tracheal strip of guinea pigs

    XUE Jun ZHANG Bing-Fang QI Hao-Wen MA Heng ZHOU Shi-Sheng ZHANG Cai-Lian

    1 Department of Respiratory Disease，Xijing Hospi-tal，

    2 Department of Physiology，Faculty of Preclini-cal Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:chloried channel blockers;tracheal strip;guinea pig;5-hydroxytryptamine;calcium anon

    Abstract:AIM To study the effects and mechanisms of chloride channel blockers(NFA and NPPB)on the contrac-tion of isolated tracheal strips of guinea pigs.METHODS The incubated isolated tracheal strips of guinea pigs were pre-pared.Various drugs were added respectively.KCl or5-HT induced contractile effects and changes in KCl or5-HT caused by chloride channel blockers and verapamil(Ver)were deter┐mined.RESULTS The contractions induced by KCl or5-HT in isolated tracheal strips of guinea Pig were markedly in-hibited by NFA and NPPB.The depressed percentage were100percent and30percent ......