关键词: Toll样受体;亮氨酸;重复序列，核酸;逆转录聚合酶链反应;克隆，分子;序列分析

    摘 要:目的 为研究TLR2胞外域在肽聚糖诱导的信号转导中的功能，构建TLR2胞外域及其氨基端和羧基端片段真核表达载体. 方法 提取HL-60细胞总RNA，以RT-PCR方法获取了TLR2胞外域cDNA片段，将其与pGEM-T Easy载体连接，转化E.coli JM109，建立了TLR2胞外域的cDNA克隆;籍此，又相继克隆了胞外域的氨基端和羧基端片段，然后将这三个片段克隆到真核表达载体pcDNA3中. 结果 序列分析表明，与GenBank中的人TLR2全长cDNA序列比较，仅4个碱基不同，同源性为0.998. 结论 成功获得了HL-60细胞TLR2胞外域及其氨基端和羧基端片段的克隆.

    Cloning and sequencing of extracellular domain and N┐Terminal and C┐Terminal fragments of Toll┐like receptor2

    ZHANG Lin，YU Wen-Bin，MA Yue-Yun，SU Ming-Quan，DING Zhen-Ruo

    Clinical Molebiology Laboratory，Department of Clinical Laboratories，Xijing Hospital，Fourth Mili-tary Medical University，Xi'an710033，China

    Keywords:Toll-like receptor;leucine;repetitive sequences，nucleic acid;reverse transcriptase polymerase chain reaction;cloning，molecular;sequence analysis

    Abstract:AIM To find out the role of the extracellular do-main of TLR2in the peptidoglycan-induced signaling path-way，mammalian expression plasmids of the extracellular do-main of TLR2and its two fragments were constructed.METHODS The cDNA fragment encoding complete extra-cellular domain was isolated by semi-nested RT-PCR method with the total RNA extracted from HL-60cells ......