【摘要】 目的 确定ESM-1mRNA在肾癌中的定位，探讨其表达在肾癌生物学特性中的意义。 方法 采用地高辛标记的原位杂交技术检测ESM-1mRNA在50例肾癌、30例癌旁正常肾组织中的表达状况，并分析其与临床病理特征的关系。 结果 ESM-1mRNA主要定位于血管内皮细胞的胞质伴胞核中，在正常肾组织与肾癌中均有表达，部分表达在肾小管上皮和肾癌细胞。肾癌间质血管内皮的ESM-1mRNA高表达率(52.00%)显著高于正常肾组织的血管内皮(13.33%);肾透明细胞癌与颗粒细胞癌中ESM-1mRNA的表达差异有显著性，而与患者的性别、年龄、肿瘤的组织学分级、淋巴结转移和肿块大小无明显相关。 结论 ESM-1mRNA表达可作为新生血管内皮的标志物，可能与肾癌的血管生成及发生、发展有关。

    【关键词】 肾肿瘤;RNA，信使;原位杂交

    In situ detection and significance of endothelial cell specific molecule ESM-1gene in renal cell carcino-ma

    XIONG Jiang-xia，WU Qiang ，ZHOU Qing，GUI Shu-yu ，WANG Yuan.

    1.Laboratory of molecular biology，Anhui Medical University，and Anhui Province Key Laboratory of Genomic Re-search，Hefei 230032，China;2.Department of Pathology，Anhui Medical University，Hefei 230032，China;3.Department of Respiratory Medicine，The First Affiliated Hospital of Anhui

    Medical University，Hefei 230022，China

    【Abstract】 Objective To observe the histological localization of ESM-1gene expression in renal cell carcinoma，and to discuss its clinical significance.Methods With in situ hybridization method，ESM-1mRNA expression was detected and their relationships with clinic pathologic features were also analyzed in50cases with renal carcinoma and in30cases with normal renal tissues adjacent to neoplastic lesions.Results ESM-1mRNA expression was found both in renal carcinoma and in normal renal tissue ......