胃癌多基因甲基化状态分析
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赵成海, 张 宁, 卜献民, 李 岩, 张海鹏
胃癌;甲基化;p16;hMLH1;E-cadherin;RUNX3赵成海,张宁,卜献民,李岩,张海鹏.胃癌多基因甲级化状态分析. 世界华人消化杂志2006;14(10)1004-1007&
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赵成海, 张宁, 张海鹏, 中国医科大学病理生理教研室 辽宁省沈阳市 110001
卜献民, 中国医科大学附属二院普通外科 辽宁省沈阳市 110004
李岩, 中国医科大学附属二院消化内科 辽宁省沈阳市 110004
通讯作者: 张海鹏, 110001, 沈阳市和平区北二马路92号, 中国医科大学病理生理教研室.zhanghp@hotmail.com
电话: 024-23256666-5291
收稿日期: 2006-02-13 接受日期: 2006-03-07
Multiple genes methylation detection in gastric cancer
Cheng-Hai Zhao, Ning Zhang, Xian-Min Bu, Yan Li, Hai-Peng Zhang
Cheng-Hai Zhao, Ning Zhang, Hai-Peng Zhang, Department of Pathophysiology, China Medical University, Shenyang 110001, Liaoning Province, China
Xian-Min Bu, Department of General Surgery, the Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Yan Li, Department of Gastroenterology, the Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Correspondence to: Hai-Peng Zhang, Department of Pathophysiology, China Medical University, Beier Road, Heping District, Shenyang 110001, Liaoning Province, China. zhanghp@hotmail.com
Received: 2006-02-13 Accepted: 2006-03-07
Abstract
AIM: To determine methylation state of p16, hMLH1, E-cadherin and RUNX3 and explore the role of these genes concurrent methylation in gastric cancer.
METHODS: DNA in gastric cancer, cancer-adjacent tissues and normal gastric mucosa was extracted by saturated NaCl method. Methylation state of these genes was detected by methylation-specific polymerase chain reaction (MSP).
RESULTS: Methylation of E-cadherin and RUNX3 was found in 38.9% and 16.7% normal gastric mucosa respectively. Methylation of p16 and hMLH1 was not present in any normal gastric mucosa. The methylation rate of p16, hMLH1, E-cadherin and RUNX3 is 8.3%, 4.2%, 54.2% and 29 ......
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